DSpace Repository

High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging

Show simple item record

dc.contributor.author Altay Arpalı, Serap
dc.contributor.author Arpalı, Çağlar
dc.contributor.author Coşkun, Ahmet F.
dc.contributor.author Chiang, Hsin-Hao
dc.contributor.author Özcan, Aydoğan
dc.date.accessioned 2017-02-28T10:53:52Z
dc.date.available 2017-02-28T10:53:52Z
dc.date.issued 2012
dc.identifier.citation Altay Arpalı, S...et al. (2012). High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging. Lab On A Chip, 12(23), 4968-4971. http://dx.doi.org/10.1039/c2lc40894e tr_TR
dc.identifier.issn 1473-0197
dc.identifier.uri http://hdl.handle.net/20.500.12416/1323
dc.description.abstract Undiluted blood samples are difficult to image in large volumes since blood constitutes a highly absorbing and scattering medium. As a result of this limitation, optical imaging of rare cells (e.g., circulating tumour cells) within unprocessed whole blood remains a challenge, demanding the use of special microfluidic technologies. Here we demonstrate a new fluorescent on-chip imaging modality that can rapidly screen large volumes of absorbing and scattering media, such as undiluted whole blood samples, for detection of fluorescent micro-objects at low concentrations (for example <= 50-100 particles/mL). In this high-throughput imaging modality, a large area microfluidic device (e.g., 7-18 cm(2)), which contains for example similar to 0.3-0.7 mL of undiluted whole blood sample, is directly positioned onto a wide-field opto-electronic sensor-array such that the fluorescent emission within the microchannel can be detected without the use of any imaging lenses. This microfluidic device is then illuminated and laterally scanned with an array of Gaussian excitation spots, which is generated through a spatial light modulator. For each scanning position of this excitation array, a lensfree fluorescent image of the blood sample is captured using the opto-electronic sensor-array, resulting in a sequence of images (e.g., 144 lensfree frames captured in similar to 36 s) for the same sample chip. Digitally merging these lensfree fluorescent images based on a maximum intensity projection (MIP) algorithm enabled us to significantly boost the signal-to-noise ratio (SNR) and contrast of the fluorescent micro-objects within whole blood, which normally remain undetected (i.e., hidden) using conventional uniform excitation schemes, involving plane wave illumination. This high-throughput on-chip imaging platform based on structured excitation could be useful for rare cell research by enabling rapid screening of large volume microfluidic devices that process whole blood and other optically dense media. tr_TR
dc.language.iso eng tr_TR
dc.publisher Royal Soc Chemistry tr_TR
dc.relation.isversionof 10.1039/c2lc40894e tr_TR
dc.rights info:eu-repo/semantics/closedAccess
dc.subject Circulating Tumor-Cells tr_TR
dc.subject Microscopy tr_TR
dc.title High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging tr_TR
dc.type article tr_TR
dc.relation.journal Lab On A Chip tr_TR
dc.identifier.volume 12 tr_TR
dc.identifier.issue 23 tr_TR
dc.identifier.startpage 4968 tr_TR
dc.identifier.endpage 4971 tr_TR
dc.contributor.department Çankaya Üniversitesi, Mühendislik Fakültesi, Elektronik ve Haberleşme Mühendisliği tr_TR


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record