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Fast fluorometric enumeration of E. coli using passive chip

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dc.contributor.author Kasap, Esin Nagihan
dc.contributor.author Doğan, Üzeyir
dc.contributor.author Çoğun, Ferah
dc.contributor.author Yıldırım, Ender
dc.contributor.author Boyacı, İsmail Haklu
dc.contributor.author Çetin, Demet
dc.contributor.author Suludere, Zekiye
dc.contributor.author Tamer, Uğur
dc.contributor.author Ertaş, Nusret
dc.date.accessioned 2020-02-07T13:00:02Z
dc.date.available 2020-02-07T13:00:02Z
dc.date.issued 2019-09
dc.identifier.citation Kasap, Esin Nagihan...et al., "Fast fluorometric enumeration of E. coli using passive chip", Journal of Microbiological Methods, Vol. 164, (2019). tr_TR
dc.identifier.issn 0167-7012
dc.identifier.uri http://hdl.handle.net/20.500.12416/2409
dc.description.abstract In this report, a passive microfluidic chip design was developed for fast and sensitive fluorometric determination of Escherichia coli (E. coli) based on sandwich immunoassay. Initially, magnetic nanoparticles (MNPs) and chitosan modified mercaptopropionic acid capped cadmium telluride (CdTe) quantum dots (QDs) were functionalized with E.coli specific antibody to form a sandwich immunoassay with the E. coli. The magnetic separation and preconcentration of the E.coli from the sample solution was performed in the vial. Conjugation of QDs to the magnetically captured E. coli and washing were performed using a passive type of microchip. The microfluidic chip consists of four microchambers connected to each other by microchannels which act as capillary valves. Signal measurement was performed at the last chamber by using a hand-held spectrofluorometer equipped with a fiber optic reflection probe. The selectivity of the method was tested with Enterobacter aerogenes (E. aerogenes) and Salmonella enteritidis (S. enteritidis), it was observed that these bacteria have no interference effect on E.coli determination. The calibration curve was found to be linear in the range of 10(1)-10(5) cfu/mL with a correlation coefficient higher than 0.99. The limit of detection was calculated as 5 cfu/mL. The method was successfully applied to spiked tap and lake water samples. The results suggest that the developed method is applicable for on-site E. coli detection and offers several advantages such as large dynamic range, high sensitivity, high selectivity and short analysis time. tr_TR
dc.language.iso eng tr_TR
dc.publisher Elsevier tr_TR
dc.relation.isversionof 10.1016/j.mimet.2019.105680 tr_TR
dc.rights info:eu-repo/semantics/closedAccess tr_TR
dc.subject E. Coil tr_TR
dc.subject Microfluidic Chip tr_TR
dc.subject Immunornagnetic Separation tr_TR
dc.subject Fluorescence tr_TR
dc.subject Sandwich Immunoassay tr_TR
dc.title Fast fluorometric enumeration of E. coli using passive chip tr_TR
dc.type article tr_TR
dc.relation.journal Journal of Microbiological Methods tr_TR
dc.identifier.volume 164 tr_TR
dc.contributor.department Çankaya Üniversitesi, Mühendislik Fakültesi, Makine Mühendisliği Bölümü tr_TR


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