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SERS-Based Ultrafast and Sensitive Detection of Luteinizing Hormone In Human Serum Using A Passive Microchip

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dc.contributor.author Gjergjizi, Belma
dc.contributor.author Çoğun, Ferah
dc.contributor.author Yıldırım, Ender
dc.contributor.author Eryılmaz, Merve
dc.contributor.author Selbes, Yeşim
dc.contributor.author Sağlam, Necdet
dc.contributor.author Tamer, Uğur
dc.date.accessioned 2020-03-26T06:25:03Z
dc.date.available 2020-03-26T06:25:03Z
dc.date.issued 2018-09-15
dc.identifier.citation Gjergjizi, Belma...et al. (2018). "SERS-based ultrafast and sensitive detection of luteinizing hormone in human serum using a passive microchip", Sensors and Actuators B-Chemical, Vol. 269, pp. 314-321. tr_TR
dc.identifier.issn 0925-4005
dc.identifier.uri http://hdl.handle.net/20.500.12416/2730
dc.description.abstract Human luteinizing hormone (LH) is an important analyte for doping control analysis since it increases the athletic performance. However, traditional methods to detect LH have few disadvantages, such as long analysis duration, waste disposal problem and sample matrix effect. Addressing these problems, surface enhanced Raman spectroscopy based LH analysis using a passive microfluidic chip was developed and optimized. Antibody modified magnetic gold nanoparticles captured the LH and then, 4-aminothiophenol (4-ATP) labeled nanoparticles formed the sandwich immunoassay structure. The complex and the other reactions occurred in different chambers of the chip. The SERS signals of 4-ATP were recorded from the chamber and the system was shown to detect 0.036 IU L-1 in serum samples. The performance of the immunoassay was compared to all other methods and the proposed assay was the fastest analysis of LH without any problems associated with the sensitivity. The shorter analysis time was recorded because the chip enables the control of all reactions in one place and there was no requirement of a specialized laboratory. (c) 2018 Elsevier B.V. All rights reserved. tr_TR
dc.language.iso eng tr_TR
dc.publisher Elsevier Science SA tr_TR
dc.relation.isversionof 10.1016/j.snb.2018.05.001 tr_TR
dc.rights info:eu-repo/semantics/closedAccess tr_TR
dc.subject Luteinizing Hormone tr_TR
dc.subject Gold Nanoparticle tr_TR
dc.subject SERS tr_TR
dc.subject Microchip tr_TR
dc.subject Immunoassay tr_TR
dc.title SERS-Based Ultrafast and Sensitive Detection of Luteinizing Hormone In Human Serum Using A Passive Microchip tr_TR
dc.type article tr_TR
dc.relation.journal Sensors and Actuators B-Chemical tr_TR
dc.contributor.authorID 3837 tr_TR
dc.identifier.volume 269 tr_TR
dc.identifier.startpage 314 tr_TR
dc.identifier.endpage 321 tr_TR
dc.contributor.department Çankaya Üniversitesi, Mühendislik Fakültesi, Mekatronik Mühendisliği Bölümü tr_TR


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