Applications of Mexican Hat Wavelet Function to Binary Mixture Analysis

Abstract

The simultaneous determination of pyridoxine hydrochloride (B6) and isoniazide (INH) in tablets was performed by using the Mexican hat wavelet function (MEXH). The absorption spectra of B6, INH and their samples were recorded in the spectral range of 200-330 nm. Linear regression functions for B6 and INH in the linear concentration range of 5-45 mu g/mL were obtained by measuring the transformed signals at 253.5 nm corresponding to the zero-crossing point for INH and at 270.6 nm corresponding to the zero-points for B6, respectively. The validation of MEXH method was performed by using the synthetic mixtures. The percent mean recoveries and their relative standard deviations were found as 98.7% and 2.06 for B6 and 98.9% and 1.89 for INH in the synthetic mixture analysis. The MEXH method was applied to the real samples and a good sensitivity and selectivity was reported. The MEXH method was sucessfully compared with classical derivative method