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A new application of chemometric techniques to HPLC data for the simultaneous analysis of a two-component mixture

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dc.contributor.author Dinç, E.
dc.contributor.author Üstündağ, O.
dc.contributor.author Özdemir, A.
dc.contributor.author Baleanu, Dumitru
dc.date.accessioned 2020-04-18T13:29:15Z
dc.date.available 2020-04-18T13:29:15Z
dc.date.issued 2005
dc.identifier.citation Dinç, E.; Üstündağ, O.; Özdemir, A.; Baleanu, D., "A new application of chemometric techniques to HPLC data for the simultaneous analysis of a two-component mixture", Journal Of Liquid Chromatography & Related Technologies, Vol.28, No.14, pp.2179-2194, (2005). tr_TR
dc.identifier.issn 1082-6076
dc.identifier.uri http://hdl.handle.net/20.500.12416/3342
dc.description.abstract A new chemometric approach using high performance liquid chromatography (HPLC) with photodiode array (PDA) detection was developed and applied to the simultaneous determination of enalapril maleate (EA) and hydrochlorothiazide (HCT) in tablets. Chernometric calibration techniques, classical least squares (CLS), principle component regression (PCR), and partial least squares (PLS) were subjected to the peak area at multiwavelength PDA detector responses. The combination of HPLC and chernometric calibration techniques was called HPLC-CLS, HPLC-PCR, and HPLC-PLS. For comparison purposes, the HPLC method called classical HPLC method was used for the confirmation of the results obtained from combined HPLC-chemometric calibration techniques. A good chromatographic separation between two drugs and internal standard (IS) was achieved using a Waters Symmetry (R) C 18 Column 5 mu m 4.6 x 250 mm and a mobile phase consisting of 0.2M acetate buffer and acetonitrile (v/v, 60:40). The multiwavelength PDA detection was done at 230 (A), 240 (B), 250 (C), 250 (D), 240 (E) nm wavelengths, and peak area was recorded for the concentration set in the mobile phase. Three HPLC-chemometric calibrations and a classical-HPLC method were tested by analyzing the synthetic mixture of EA and HCT in the presence of losartan potassium (IS). The proposed methods were applied to real samples containing the present two drugs. The obtained results were statistically compared with each other. tr_TR
dc.language.iso eng tr_TR
dc.publisher Taylor&Francis tr_TR
dc.relation.isversionof 10.1081/JLC-200064041 tr_TR
dc.rights info:eu-repo/semantics/closedAccess tr_TR
dc.subject HPLC-Chemometric Calibration tr_TR
dc.subject Multiwavelength HPLC Data Processing tr_TR
dc.subject Quantitative Determination tr_TR
dc.subject Enalapril Maleate tr_TR
dc.subject Hydrochlorothiazide tr_TR
dc.subject Pharmaceutical Dosage Form tr_TR
dc.title A new application of chemometric techniques to HPLC data for the simultaneous analysis of a two-component mixture tr_TR
dc.type article tr_TR
dc.relation.journal Journal Of Liquid Chromatography & Related Technologies tr_TR
dc.contributor.authorID 56389 tr_TR
dc.identifier.volume 28 tr_TR
dc.identifier.issue 14 tr_TR
dc.identifier.startpage 2179 tr_TR
dc.identifier.endpage 2194 tr_TR
dc.contributor.department Çankaya Üniversitesi, Fen Edebiyat Fakültesi, Matematik Bölümü tr_TR


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