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Determination of guaifenesin and dextromethorphan in a cough syrup by HPLC with fluorometric detection

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dc.contributor.author Özdemir, Abdil
dc.contributor.author Aksoy, Halil
dc.contributor.author Dinç, Erdal
dc.contributor.author Baleanu, Dumitru
dc.contributor.author Dermiş, Saadet
dc.date.accessioned 2016-03-31T12:24:06Z
dc.date.available 2016-03-31T12:24:06Z
dc.date.issued 2006-02
dc.identifier.citation Özdemir, A..et al. (2006). Determination of guaifenesin and dextromethorphan in a cough syrup by HPLC with fluorometric detection. Revue Roumaine De Chimie, 51(2). 117-122. tr_TR
dc.identifier.issn 0035-3930
dc.identifier.uri http://hdl.handle.net/20.500.12416/798
dc.description.abstract A practical high-performance liquid chromatographic method with fluorometric detection for the determination of guaifenesin and dextromethorphan in cough syrup is described. The developed method contains simple, accurate and rapid sample preparation steps for the separation and simultaneous determination of active compounds in samples with existence of other excipients. Amilorid (AM) was used as an internal standard (IS) that has a fluorescence character in the working wavelength region. A good chromatographic separation of two drugs and IS was achieved on a Waters Symmetry(R) C18 Column 5 mu m 4.6 x 250 mm with mobile phase consisting of phosphate buffer (0.2 M, pH=2), acetonitrile and methyl alcohol (v/v, 62:23:15). Eluents were monitored by fluorescence detection at the excitation and emission wavelengths of 277 and 588 nm, respectively. Under the described conditions, the obtained calibration graphs are linear over the concentration range of 0.054.2 and 1.2-2.4 mg/mL for dextromethorphan and guaifenesin, respectively and the regression coefficients were greater than 0.999. The validation study was carried out fulfilling the ICH guidelines in order to prove that the new analytical method meets the fundamental criteria including selectivity, linearity, precision, accuracy and sensitivity. tr_TR
dc.language.iso eng tr_TR
dc.publisher Edıtura Academiei Romane tr_TR
dc.rights info:eu-repo/semantics/closedAccess
dc.subject Liquid-Chromatography tr_TR
dc.subject Gas-Chromatography tr_TR
dc.subject Human Plasma tr_TR
dc.subject Pseudoephedrıne tr_TR
dc.subject Metabolıtes tr_TR
dc.subject Serum tr_TR
dc.title Determination of guaifenesin and dextromethorphan in a cough syrup by HPLC with fluorometric detection tr_TR
dc.type article tr_TR
dc.relation.journal Revue Roumaine De Chimie tr_TR
dc.contributor.authorID 26096 tr_TR
dc.contributor.authorID 6981 tr_TR
dc.contributor.authorID 169890 tr_TR
dc.identifier.volume 51 tr_TR
dc.identifier.issue 2 tr_TR
dc.identifier.startpage 117 tr_TR
dc.identifier.endpage 122 tr_TR
dc.contributor.department Çankaya Üniversitesi, Fen Edebiyat Fakültesi, Matematik Bilgisayar Bölümü tr_TR


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