Silicon-based core-shell nanoparticle's nanobiomedical characterisation

Abstract

Using functionalised nanoparticles in nanobiomedical applications cause to raise questions about unintentional effect of such powerful agents on the human body. In other words, the study of nanoparticle toxicity can be considered as a curtail key for the biological applications. In this study, the core/shell nanoparticles such as Si/Au and SiO2/Au were synthesised and functionalised with some biological elements. For satisfying some medical standards and biomedical critical test conditions, a few in-vitro assays as cytotoxicity and haemolysis should be done. For this reason, cytotoxicity and haemolytic effects of the functionalised nanoparticles such as Si/Au/biotin and SiO2/Au/biotin and their derivatives were evaluated on Hep-G2 cells and humane red blood samples. It is shown that cytotoxicity and haemolysis effects of the all synthesised nanoparticles are concentration-dependent. Also, the results are shown that most cytotoxicity and haemolytic effects are observed for Si/Au and SiO2/Au without biotin groups after 48 hours and 30 minutes, respectively.