Continuous wavelet transform applied to the overlapping absorption signals and their ratio signals for the quantitative resolution of mixture of oxfendazole and oxyclozanide in bolus

Abstract

Continuous wavelet transform (CWT) was applied to the overlapping absorption signals and their ratio signals for the quantitative resolution of mixture of oxfendazole (OXF) and oxyclozanide (OXY) in bolus without using any pre-chemical treatment. Mexican hat function (MEXH) and Morlet (MORL) continuous wavelet transforms (MEXH-CWT and MORL-CWT) for signal treatments were found to be suitable among the wavelet families. In the first step MEXH-CWT together with zero crossing technique is directly applied to the overlapping absorption spectra of OXF and OXY drugs and their calibration graphs were obtained by measuring the MEXH-CWT amplitudes at 328.7 nm for OXY and at 309.3 nm for OXF in the wavelet domain of 255-365 nm. In the second step MORL-CWT is used for finding the significant peak resolution of the ratio spectra of OXY and OXF respectively. The maxima and minima of CWT amplitudes and peak-to-peak and zero-crossing techniques were used for the calibration graphs. The method performances of CWT-zero crossing and ratio spectra-CWT techniques were validated by analyzing the synthetic mixtures and by applying the standard addition technique, and successfully accuracy, precision and reproducibility results were reported